THli in the buccal nervous system.

(A1) Ventral view of the right buccal hemiganglion. Outline of the ganglion is marked by the dashed blue line. THli fibers coursed through the ventral commissure (VC) and through the buccal neuropil. The majority crossed the ganglion toward buccal root (R3) but several fibers projected into the first buccal root (R1). Three neurons were stained on the anterior margin of the immunoreactive fiber tract projecting into R3. The two medial neurons were contiguous (arrow). (A2) Ventral view of the left buccal hemiganglion. Four neurons were present in the medial region of the ganglion, including a medial pair (arrow) and a lateral pair (arrowhead). There was no immunoreactivity on the dorsal surface of the ganglion. Calibration bar = 100 μm, applies to A1 and A2. (B1) Higher magnification of the medial pair of THli cells in the left buccal hemiganglion. Both cells had two fibers originating from the soma, one of which projected medially (arrows) while the other projected laterally (arrowheads). Calibration bar = 40 μm. (B2) Anterolateral region of left buccal hemiganglion, ventral view. Two stout axons were present in the cerebrobuccal connective (CBC). Calibration bar = 100 μm. (B3) A single THli neuron was located in the stomatogastric ganglion (STG), which is demarcated by the dashed blue line. Two fibers originated from the cell soma (arrowheads), one projecting toward the periphery via the lateral gastroesophegeal nerve (LGEN) and the other toward the CNS via the stomatogastric nerve (SGN). Calibration bar = 100 μm.