Efp silencing increases 14-3-3σ protein levels in endometrial cancer cells.

(A) Knockdown efficiency of Efp siRNAs in Ishikawa cells. Cells were transfected with siEfp #A, #B, or negative control siControl for 48 h. Efp mRNA levels were determined by qRT-PCR and normalized to 36B4 levels. (B) Efp siRNAs decreased Efp protein levels and increased 14-3-3σ protein levels in Ishikawa cells. Whole cell lysates were prepared from Ishikawa cells transfected with siEfp #A, #B, or siControl, and then subjected to western blot analysis using antibodies for Efp and 14-3-3σ. β-actin levels were analyzed as a loading control. (C) Knockdown efficiency of Efp siRNAs in HEC-1A cells. Experiments were performed using HEC-1A cells as in (A). Data are presented as means ± s.d. (n = 3). **, P D) Efp siRNAs decreased Efp protein levels and increased 14-3-3σ protein levels in HEC-1A cells. Experiments were performed using HEC-1A cells as in (B).