Decrease of Th1/Th17 markers after P28GST treatment and predominance of Alternatively Activated Macrophages.

Mice were treated with 2 ip injections of either P28GST or methylprednisolone after TNBS injection. Colons were removed after sacrifice2 days after TNBS injection. Tbet (A), RORγ (B) and GATA3 (C) respectively markers of T helper (Th) 1, Th17 and Th2 cells, and Arg and iNOS (D), respectively markers of M2 and M1 macrophages, mRNA expression was assessed by quantitative PCR in mice colons. Results are expressed as means ± SEM from four similar experiments, n = 10–12 mice per group. *p<0.05; **p<0.01; ***p<0.001.