A 98,493 base-pair region of the MTM3W5.2 genome contains the proposed biosynthetic gene cluster required to produce the inhibitory compound.

Large arrows and arrow heads indicate proposed genes with numbers designating the specific BTZ20 loci of the genome sequence. The gene cluster likely begins with the regulatory locus BTZ20_3964 (at position 3894940) and may end with the three small loci BTZ20_3939–37 (at position 3796447). This includes a possible NRPS gene BTZ20_3940. The two transposon insertion mutations (RMP 2.31 and RMP 77.23) are shown and map to the same large type I PKS gene BTZ20_3962. These two mutations render a phenotype that no longer produces the inhibitory compound. The dashed line shows the right end of a large (232,272 bp) postulated genome island region that may overlap with the very end of the biosynthetic gene cluster. The open rectangle represents the genomic DNA region containing the gene cluster 46. The color key is: Blue arrows are type I PKS genes, green arrow in a transcriptional regulatory gene, red arrows are NRPS type genes, and brown arrows are other gene classes.