Decreased repair temperature exhibits differential effects on repair of prompt DSB and HSCS.

GM5758 cells were irradiated with 40 Gy gamma radiation and allowed to repair at 13°C (A), 15°C (B) and 37°C (C) for the indicated time. Data represented as 37°C is taken from Fig 1A. After repair (0–4 h), chromosomal DNA was extracted and treated at 4°C or 50°C, and DSB was measured via pulsed-field gel electrophoresis. The blue lines represent repair of prompt DSBs (samples lysed in 4°C), while the red lines represent repair of HSCS and prompt DSBs (samples treated at 50°C). The error bars represent the standard deviation from 2–4 independent experiments.