Figure 8. Comparison of naïve iPSCs generation under microfluidics and conventional culture conditions.

(a) Reprogramming protocol in conventional culture conditions (CCC) with HFF-1 fibroblast cell line. At day 12 in well, a mixed population of naïve- and primed-like colonies emerged (red and blue circles). The right panel shows the typical morphology of primed- and naïve-like colonies. (b) Conversion of primed H9 into naïve pluripotent colonies in both µF and CCC using DOX-inducible NANOG-KLF2 transgenes in presence of 2iL. After 14 days colonies were stained for the naïve-specific marker TFCP2L1. Under µF conditions we observed a drastic increase in colony formation. Scale bars 100 µm (a, b), 1 mm (a, tiling)