C was activated in-vitro via the CP by aggregated IgG (A) or via the AP by Zymosan (B) and levels of sC5b-9 were determined. Basal sC5b-9 levels were subtracted. Values are given as mean with SEM. NC (white bars): n = 13; CLL with normal C5 (grey bars): n = 23; CLL with abnormal C5 (black bars): n = 16. *p = 0.002 vs. NC. (C) The correlation between sC5b-9 levels at baseline and after CP activation was analyzed in all subjects. NC (white circles): n = 13; CLL with normal C5 (grey circles): n = 23; CLL with abnormal C5 (black circles): n = 16.</p
By in vitro complement immunofluorescence, 6 sera from pemphigus with intercellular antibodies were ...
<p>For measuring the background, classical, lectin & alternative and only alternative pathway activa...
Nd a Cy5-conjugated antibody for C1q, C3, C7 or C5b-9, or appropriate IgG controls before analyzed o...
Basal levels of C activity markers were determined without activation. (A) The levels of sC5b-9 were...
C5-deficient serum was supplemented with 33% serum from CLL or NC or with purified C5 (positive cont...
Subjects’ sera were supplemented with purified C5 (commercial) to physiological concentration. C act...
C activation was inhibited in subjects’ sera by EDTA. Sera were then supplemented with classical C5-...
Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in the Western world. The thera...
Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in the Western world. The thera...
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in ...
The potential identity of the abnormal C5 as C5b was assessed by Western analysis using anti-C5 and ...
Background Complement activation plays an important pathogenic role in numerous diseases. The ratio...
Therapy regimens for Chronic lymphocytic leukemia (CLL) commonly include chemotherapy and immunother...
<p>Two μg of human C1q was pre-incubated with increasing amounts of r<i>T</i>s-Pmy (0, 2, 4 μg) or B...
Complement C5 component was followed in plasma under non-reducing conditions, by Western analysis us...
By in vitro complement immunofluorescence, 6 sera from pemphigus with intercellular antibodies were ...
<p>For measuring the background, classical, lectin & alternative and only alternative pathway activa...
Nd a Cy5-conjugated antibody for C1q, C3, C7 or C5b-9, or appropriate IgG controls before analyzed o...
Basal levels of C activity markers were determined without activation. (A) The levels of sC5b-9 were...
C5-deficient serum was supplemented with 33% serum from CLL or NC or with purified C5 (positive cont...
Subjects’ sera were supplemented with purified C5 (commercial) to physiological concentration. C act...
C activation was inhibited in subjects’ sera by EDTA. Sera were then supplemented with classical C5-...
Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in the Western world. The thera...
Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in the Western world. The thera...
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in ...
The potential identity of the abnormal C5 as C5b was assessed by Western analysis using anti-C5 and ...
Background Complement activation plays an important pathogenic role in numerous diseases. The ratio...
Therapy regimens for Chronic lymphocytic leukemia (CLL) commonly include chemotherapy and immunother...
<p>Two μg of human C1q was pre-incubated with increasing amounts of r<i>T</i>s-Pmy (0, 2, 4 μg) or B...
Complement C5 component was followed in plasma under non-reducing conditions, by Western analysis us...
By in vitro complement immunofluorescence, 6 sera from pemphigus with intercellular antibodies were ...
<p>For measuring the background, classical, lectin & alternative and only alternative pathway activa...
Nd a Cy5-conjugated antibody for C1q, C3, C7 or C5b-9, or appropriate IgG controls before analyzed o...