Identification of four genes from the porcupine microbiome with putative cellulose- and/or hemicellulose-degrading activity using a metagenomic sequencing pipeline.

The bioinformatic pipeline (A) began with Illumina MiSeq data previously collected from a porcupine fecal DNA sequencing project [14]. Reads were checked for quality and trimmed, concatemerized via MegaHit [15], and open reading frames were identified using Prodigal [16]. Protein sequences of interest were identified by pHMMER [17] using various protein databases and were selected for matches of interest based on e-value selection. (B) Top putative microbial enzymes identified by the metagenomic sequencing pipeline; putative signal sequences are shown in orange and predicted conserved protein domains are shown.