Additional file 3: of N-acetylcysteine decreases malignant characteristics of glioblastoma cells by inhibiting Notch2 signaling

Figure S3. NAC causes G1 arrest in GBM cells. A, The cell cycle analysis by measuring the percentage of cells in each phase using flow cytometry in U87 and U251 cells. B, The western blot analysis of P21, cyclin E and CDK2 in U87 and U251 cells. All cells were electroporated with pcDNA3.1-Notch2 or pcDNA3.1-EV, pcDNA3.1-EV served as a control, followed by BSO (1 mM, 12 h) and NAC (10 mM, 24 h) treatment. β-actin was used as a loading control. All data are presented as means ± SD of three independent experiments. * P < 0.05 compared with EV group, # P < 0.05 compared with EV + NAC + BSO group. (TIF 5721 kb