Additional file 8: of The peacefulness gene promotes aggression in Drosophila

Figure S4. cin knockdown did not affect physical capabilities. (A) Climbing test. The percentage of flies that crossed the 10-cm mark after 15-s climbing was quantified. Number of trials: nSyb-Gal4/+, n = 27; cinKK102795/+, n = 33; nSyb-Gal4/cinKK102795, n = 36; cinHMS00420/+, n = 24; nSyb-Gal4/+;cinHMS00420/+, n = 24. No significant difference was observed between knockdown flies and control flies (Kruskal-Wallis and post hoc Mann-Whitney U tests, “ns”, not significant, P > 0.05). (B) Locomotor activity for 15-min period. Total travel distance for 15-min period was quantified. Number of individual flies tested: nSyb-Gal4/+, n = 42; cinKK102795/+, n = 48; nSyb-Gal4/cinKK102795, n = 50; cinHMS00420/+, n = 44; nSyb-Gal4/+;cinHMS00420/+, n = 46. Kruskal-Wallis and post hoc Mann-Whitney U tests, ***P 0.05. Error bars represent SEM. (PDF 180 kb