Sperm aneuploidy assessed by NGS in specimens of different origins with related pregnancy outcome.

The two-sample t-test and Mann-Whitney U test were used to compare the autosomal and gonosomal aneuploidy rates assessed by NGS in at least 1000 spermatozoa and evidenced that the ejaculated (n = 16) specimen had a higher incidence of aneuploidy than the epididymal (n = 2), testicular (n = 4), and donor control specimen (n = 2) (P<0.0001) (Upper graph). In the lower graph, the clinical pregnancies and their evolution into miscarriage or term, assessed by Friedman’s Chi-square test, are represented according to the source of spermatozoa used for ICSI. No statistical difference was found in this analysis.