Results of anion-exchange chromatography obtained by purification of conjugation reaction products involving an antibody with Ab-N3 DOL = 1.1.
- Artem V. Maerle (6175631)
- Maria A. Simonova (2057230)
- Victor D. Pivovarov (6175634)
- Daria V. Voronina (6175637)
- Polina E. Drobyazina (6175640)
- Dmitriy Yu. Trofimov (6175643)
- Leonid P. Alekseev (6175646)
- Sergei K. Zavriev (6175649)
- Dmitriy Yu. Ryazantsev (6175652)
Publication date
January 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(a) Anion-exchange chromatogram of conjugation reaction products involving an antibody with Ab-N3 DOL = 1.1. The numbers of fractions are indicated on the enlarged fragment (fraction 1: unreacted antibody; fractions 2–6: peak 11–12.5 min). Right: SDS-PAGE in reducing (b) and non-reducing (c) conditions. The sample numbers correspond to the fractions, MA2 is the original antibody. L: protein ladder (b: PageRuler Prestained Protein Ladder, 10 to 180 kDa; c: Spectra Multicolor Broad Range Protein Ladder).</p
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