Additional file 3: of Star-related lipid transfer protein 10 (STARD10): a novel key player in alcohol-induced breast cancer progression

Figure S1. Ethanol-induced p65 that increaseS STARD10 and ERBB2 expression in SKBR-3 cells. (A) SKBR-3 cells were treated with 100 mM ethanol and transfected with STARD10 and/or ERBB2 for 48 h. STARD10 and ERBB2 mRNA and protein levels were accomplished using RT-PCR and Western blot analysis, respectively, compared to control from 4 independent experiments. *p < 0.05 vs. EV. (B) mRNA levels of STARD10 and ERBB2 in SKBR-3 cells treated with ethanol (100 mM) and transfected with STARD10 overexpression vector and ERBB2 siRNA (10 nM) for 48 h. Results are expressed as fold relative to Sc + EV (mean ± SE) from 3 independent experiments. *p < 0.02 vs.Sc + EV; †p < 0.01 vs. STARD10; ‡p < 0.05 vs. EtOH. (C) STARD10 and ERBB2 promoter activity analysis was performed using reporter assay from 4 independent experiments. *p < 0.02 vs. EV STARD10 promoter; *p < 0.04 vs. EV ERBB2 promoter. (D) Cells were treated with 100 mM ethanol or transfected with p65 plasmid for 48 h. STARD10 and ERBB2 expression was analyzed by RT-PCR and Western Blotting analysis to measure their mRNA and protein levels. Results are expressed as fold relative to EV (mean ± SE) from 3 independent experiments. *p < 0.05 vs. EV mRNA; *p < 0.03 vs. EV proteins. (PPTX 69 kb