Knockdown of RASSF1A facilitates G1 to S transition by upregulating CyclinD1 and Cyclin E expression in LCL cells.

A-B) RASSF1A knocked down BJAB and LCL1 cells were stained with PI staining buffer and analyzed by flow cytometry. Histogram showed the average values of two independent experiments. C-D) The expression of cyclin proteins were detected in RASSF1A knocked down BJAB and LCL1 cells. 10 million indicated cells were harvested and lysed for western blot. The expression of RASSF1A and cyclin proteins were detected by specific antibodies. The relative density (RD) of Cyclin D1 and Cyclin E were quantitated and shown.