Alteration in the cytotoxic program of HLA-DR/CD38-expressing CD8⁺ T-cells from HIV-infected patients.

Frequencies of HLA-DR/CD38-expressing CD8+ T-cells that are CD107a+ (A), granzyme B+ perforin+ (B) and granzyme B+ IFN-γ+ (C) in seronegative (n = 15) and HIV-infected (n = 30) individuals. The P value of the Mann-Whitney test is shown. NS: Not statistically significant. D. Cell ACCENSE t-SNE plots of HLA-DR, CD38, perforin, granzyme B, CD107a and IFN-γ expression in merged stimulated CD8+ T-cells from representative seronegative and HIV-infected individuals (n = 4 in each group; M = 60,000 cells). The circles indicate the cells positive for the indicated molecules. E-H. Correlation between the frequency of granzyme B+ perforin+ or CD107a+ cells and the frequency of their respective total HLA-DR+ CD38- (E and F) or HLA-DR- CD38+ (G and H) CD8+ T-cells from HIV-infected individuals (n = 30). The rho and P value of the Spearman test are shown.