Additional file 1: of Sirt6 regulates efficiency of mouse somatic reprogramming and maintenance of pluripotency

Figure S1. (A): Sirt6 mRNA level after RA induced JM8A3 ES cells differentiation. All gene mRNA levels were measured by real time PCR; Oct4, Sox2 were represented as positive control, GAPDH was used as reference gene. All data were shown as mean values ± SD from three independent experiments. (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001). (B): Two CRISPR sgRNAs were designed to knockout region between exon4 and exon5 (highlighted in yellow) (781 bp); PCR primers and fragment used for genotyping was shown on the top. (C): Genotyping of the Sirt6 knockout JM8 ES clones by PCR; One pure knockout clone (D12) was selected. (D): Western blot to validate the expression of Sirt6 in Sirt6 knockout JM8 ES cells. (JPG 542 kb