Acute restraint stress induces long lasting RNA changes in the BLC.

(A) A summary of the global transcriptome profile of the BLC in mice that underwent a single acute restraint session 30 days prior. (B) A volcano plot of the RNA-seq data with significantly changed genes colored in red and unchanged genes colored in gray. (C) qPCR validation of a subset of genes significantly upregulated in the RNA-seq. *p<0.05. Error +/- s.e.m. (D+E) Ingenuity Pathway Analysis of the global transcriptional profile identified the top 10 most significant canonical pathways (D) and functional annotations (E) of the gene list. Upregulated pathways are shown in green and downregulated in orange, with the number of molecules belonging to each pathway indicated to the right.