Additional file 2: of CYR61 triggers osteosarcoma metastatic spreading via an IGF1Rβ-dependent EMT-like process

Figure S2. CYR61 controls osteosarcoma cell phenotype in vitro. (A) Morphology of K7 M2 and U2OS osteosarcoma control, CYR61 silenced and CYR61 overexpressing cells, grown in medium supplemented with 10% Fetal Calf Serum. (B) Correlation between the relative maximal cell length of K7 M2 and U2OS cells. Results are expressed as mean ± standard deviation (n > 2000 cells/field; at least 8 field/condition; experiments repeated twice). (C) Brightfield imaging of osteo- spheroids. (D) Quantitative evaluation of the relative aggregate surface. Results are expressed as mean ± standard deviation (n = 6). a: p < 0.05 vs. control cells. (E) Relative K7 M2 cell number adherent to the indicated surface, after 30 min incubation. Results are expressed as Log2 of fold change (mean ± standard deviation). a: p < 0.05 vs. control cells. (PPTX 3906 kb