Effect of inserted disulfide bridge on BCA II unfolding.

Dependence of fluorescence lifetimes τ1 (A) and τ2 (B) on urea concentration of wild-type BCA II (■ WT) and its three mutant forms with disulfide bridge A53C-A76C (), A154C-S181C () and D188C-K211C (). (C)–interdependence of two fluorescence lifetime components τ1 and τ2 of wild-type BCA II and its three mutant forms with inserted disulfide bridge. The relative deviations for τ1 and τ2 were ≤3% and ≤7% correspondingly.