Co-localization analysis of SNX6 with endogenous, constitutively active, inactive and wild-type Rab32.

(A) IHKE-1 cells were cultured on glass cover slips for 24 hours, fixed and subsequently stained with antibodies against Rab32 (cy3, red channel) and SNX6 (cy2, green channel). Blue: DAPI staining of the nucleus. (B) IHKE-1 cells were either transiently transfected with plasmids encoding GFP-Rab32 T39N or stably expressing GFP Rab32WT or GFP-Rab32 Q85L, fixed and subsequently stained with an antibody against SNX6. GFP = green channel; SNX6 (cy3) = red channel (C) HeLa cells cultured on glass cover slips were transfected with constructs encoding GFP-SNX6 or DsRed-Monomer-Rab32 wt. After 24h cells were fixed and analyzed by flourescece microscopy. GFP = green; DsRed-Monomer = red. Arrows indicate co-localization, scale bar = 10μm.