(A) gRNAs against EGFP and control gRNA were transfected into epimastigote of Cas9 cell line. The pictures were taken at 1 day after electroporation. Scale bars: 10 μm. (B) gRNAs against EGFP and control gRNA were transfected into EA derived from Cas9-ama cell line. The pictures were taken at 1 day after electroporation. Scale bars: 10 μm.</p
UMI validation screen, original data from in vivo tumor suppressor CRISPR/Cas9 screening experiment....
A fluorescence marker mOrange was inserted to the popular pLentiCrispr-V2 to create pLentiCrispr-V2-...
<p>Unsynchronized HCT116-19 cells were electroporated at a concentration of 5x10<sup>5</sup> cells/1...
<p>Unsynchronized or synchronized and released cells were transfected via electroporation with CRISP...
<p>Left-hand panel: MM cell lines were electroporated with an expression vector for EGFP (pEGFP-N3; ...
<p>INA-6-EGFP cells were electroporated with a solution containing a stealth siRNA targeting EGFP as...
<p><b>A.</b> mESC cell line L10a-GFP, in which one Rosa26 locus expresses GFP. <b>B.</b> Experimenta...
<p>a) Schematic illustrating the position of two adjacent gRNAs directed to the HIV-1 LTR. b) A time...
The human genome encodes over 80 members of the tripartite motif (TRIM) protein family. Many of the ...
(A) Kyse30 cells were transfected with EGFR-specific siRNAs (pool of n = 4 siRNAs) (EGFR KD), siRNA ...
<p>Transfection of AMO-1 cells across a range of voltages using an expression vector for EGFP (pEGFP...
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a lentiviral...
<p>(<b>A</b>) Immunofluorescent images of MCF10A cells transfected with the indicated siRNA species,...
CRISPR/Cas is an adaptive immune system in bacteria and archaea that has recently been exploited for...
<p>(A) Schematic of the <i>EGFP</i> gene in a recombinant adenoviral genome with the target sites of...
UMI validation screen, original data from in vivo tumor suppressor CRISPR/Cas9 screening experiment....
A fluorescence marker mOrange was inserted to the popular pLentiCrispr-V2 to create pLentiCrispr-V2-...
<p>Unsynchronized HCT116-19 cells were electroporated at a concentration of 5x10<sup>5</sup> cells/1...
<p>Unsynchronized or synchronized and released cells were transfected via electroporation with CRISP...
<p>Left-hand panel: MM cell lines were electroporated with an expression vector for EGFP (pEGFP-N3; ...
<p>INA-6-EGFP cells were electroporated with a solution containing a stealth siRNA targeting EGFP as...
<p><b>A.</b> mESC cell line L10a-GFP, in which one Rosa26 locus expresses GFP. <b>B.</b> Experimenta...
<p>a) Schematic illustrating the position of two adjacent gRNAs directed to the HIV-1 LTR. b) A time...
The human genome encodes over 80 members of the tripartite motif (TRIM) protein family. Many of the ...
(A) Kyse30 cells were transfected with EGFR-specific siRNAs (pool of n = 4 siRNAs) (EGFR KD), siRNA ...
<p>Transfection of AMO-1 cells across a range of voltages using an expression vector for EGFP (pEGFP...
Mouse embryonic fibroblasts (MEF) stably expressing Flag-Cas9-EGFP were transduced with a lentiviral...
<p>(<b>A</b>) Immunofluorescent images of MCF10A cells transfected with the indicated siRNA species,...
CRISPR/Cas is an adaptive immune system in bacteria and archaea that has recently been exploited for...
<p>(A) Schematic of the <i>EGFP</i> gene in a recombinant adenoviral genome with the target sites of...
UMI validation screen, original data from in vivo tumor suppressor CRISPR/Cas9 screening experiment....
A fluorescence marker mOrange was inserted to the popular pLentiCrispr-V2 to create pLentiCrispr-V2-...
<p>Unsynchronized HCT116-19 cells were electroporated at a concentration of 5x10<sup>5</sup> cells/1...