Additional file 3: of Mesenchymal stem cells alleviate the early brain injury of subarachnoid hemorrhage partly by suppression of Notch1-dependent neuroinflammation: involvement of Botch

Effects of DAPT, Notch1 siRNA and BMSCs on (Nuclear factor-κB) NF-κB phosphorylation after SAH. DAPT administration inhibited p-NF-κB expression at 24 h post-SAH. Representative western blot bands (A) and quantification of p-NF-κB in the left cortex (B) indicate that DAPT inhibited NF-κB phosphorylation at 24 h post-SAH; n = 6 in each group; data are expressed as the mean ± SEM. *P < 0.05 versus Sham, ###P < 0.001 versus SAH + DMSO group. Notch1 siRNA injection inhibited phosphorylation of NF-κB at 24 h post-SAH. Representative western blot bands (C) and quantitative analyses of p-NF-κB expression in the left cortex (D) show that knockdown Notch1 inhibited NF-κB phosphorylation at 24 h post-SAH; n = 6 in each group; data are expressed as the mean ± SEM. *P < 0.05 versus Sham, ###P < 0.001 versus SAH + Scramble siRNA group. BMSCs treatment suppressed NF-κB phosphorylation at 24 and 72 h post-SAH. Western blot data showed that BMSCs significantly suppressed the phosphorylation of NF-κB (E and F) at 24 and 72 h post-SAH; n = 6 in each group; data are expressed as the mean ± SEM. *P < 0.05 versus Sham, #P < 0.05 versus SAH + PBS group. (TIF 4601 kb