Crb subcellular accumulation in <i>apn</i>^<i>1</i> mutant tracheal cells.

Projections of confocal sections of second instar tracheal tubes of transgenic lines expressing the respective YFP-tagged Rab protein stained for Crb (magenta) or YFP (green). (A”, B”, C”) show magnifications of the boxed area in (A-A’, B-B’, C-C’), respectively. (A, A’) apn1 mutant tracheal tubes immunostained for Crb and Rab5-YFP. (A”) Magnification shows vesicular Crb, most of which does not colocalize with Rab5. (B, B’) apn1 mutant tracheal tubes immunostained for Crb and Rab11-YFP. Magnification (B”) shows vesicular Crb, most of which does not colocalize with Rab11. (C, C’) apn1 mutant tracheal tubes immunostained for Crb and Rab7-YFP. Magnification (C”) shows vesicular Crb colocalization with Rab7 of approx. 25%. Scale bars: A, A’, B, B’, C, C’: 20μm and A”, B”, C”: 5μm.