Targeted deletion of genomic regions implicated in
- Jennifer E. Chung (6208400)
- Wendy Magis (219696)
- Jonathan Vu (6208403)
- Seok-Jin Heo (46279)
- Kirmo Wartiovaara (81848)
- Mark C. Walters (523849)
- Ryo Kurita (393269)
- Yukio Nakamura (39120)
- Dario Boffelli (219692)
- David I. K. Martin (219700)
- Jacob E. Corn (21889)
- Mark A. DeWitt (2251165)
Publication date
January 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
A) Schematic depicting two targeted regions: the naturally-occurring Corfu HPFH deletion and the 1.7 kb putative repressor region (PRR) upstream of HBD, with the 2 pairs of cuts attempted shown (13). B) PCR amplification after gene editing with pairs of Cas9 RNPs that delete the Corfu and PRR regions with primers outside the targeted regions. Deletion is indicated by the presence of bands with greater mobility corresponding to the deleted alleles which give rise to shorter PCR products.</p
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