Quantitative electrophoretic multimer analysis of wildtype and mutant VWF.

(A) High-resolution agarose gel of multimer samples. Numbers beneath bands indicate the multimer size i (i = 1 corresponds to a dimer). (B-F) Frequencies of molecules of size i, as determined from the gel (for more details, see Materials and Methods and S4 Fig), for wtVWF (B) and mutants p.Cys1099Tyr (C), p.Cys2771Arg (D), p.Cys2773Arg (E), and p.Cys2811Ala (F). Insets in panels B and C show linear fits to the frequency data represented in logarithmic space, yielding values for the extent p of multimerization of 0.47 and 0.15 for wtVWF and mutant p.Cys1099Tyr, respectively.