Inhibition of either AMPK or SIRT-1 activity abrogates AD-mediated preconditioning on infarct mass extension.

A. Upper panel. Quantification of necrotic tissue, expressed as percentage of the left ventricular mass, in hearts exposed to various treatments at the end of the reperfusion interval. Statistical differences between groups were evaluated by one-factor ANOVA followed by Bonferroni correction. Values are expressed as mean ± SEM (**p A. Lower panel. Representative images of left ventricular sections quantified in panel above are shown. Hearts were cut into transverse slices and stained with TTC to differentiate necrotic (unstained) from viable (red) myocardium. B. SIRT-1 activity measured as arbitrary fluorescence units (AFU) per min, normalized to the amount of total protein in each sample (AFU/min/μg protein), in hearts exposed to various treatments at the end of the reperfusion interval. Statistical significance between groups was evaluated by one-factor ANOVA followed by Bonferroni correction. Values are expressed as mean ± SEM (*p < 0.05. **p < 0.01).