PG increased the number of necrotic cells in HepJ5 cells.

The percentage of viable and necrotic cells was measured by high-content analysis at specific time intervals. (A) PG exposure caused a dramatic decrease in HepJ5 cell viability after 24 h. (B) PG caused an increase in the number of necrotic cells after a 24-h exposure. (C) Different PG doses did not induce early apoptosis. (D) The number of late apoptotic cells was increased after exposure to 50 μM PG in HepJ5 cells. The data are presented as the mean±SD of three independent experiments in triplicate.