Additional file 1: of An ancient role for collier/Olf/Ebf (COE)-type transcription factors in axial motor neuron development

Figure S2. Distribution analysis of Ebf2-expressing motor neurons along the medio-lateral axis of the MMC. A-B: All MMC nuclei were labeled with the Lhx3 marker (red nuclear signal) in Ebf2lacZ/+ spinal cords at e12.5 (N = 3). The position of Ebf2-expressing motor neurons (anti-bGal, green signal) within the MMC column was analyzed along the medio-lateral axis at three specific rostro-caudal regions of the spinal cord (rostral brachial, caudal brachial, thoracic). Below each image, arbitrary units (a. u.) of bGal fluorescence intensity are shown along the medio-lateral axis of the MMC (see Methods). C: Schematic summary of data shown in panel B. At rostral brachial and thoracic regions, Ebf2 positive cells are mainly located at the center of the MMC column, whereas at caudal brachial regions, Ebf2 motor neurons are mainly located medially within the MMC. (PDF 4639 kb