Truncations in the C-terminus of FMDV VP1 affect capsid precursor processing.

a) Constructs encoding the FMDV P1-2A (wt) were modified to introduce truncations at different sites in the VP1 coding sequence. The 2A L1Stop truncation served as a second positive control for processing. b) Cell lysates from BHK cells (infected with vTF7-3 [45]) transfected with plasmids that express the FMDV P1-2A (wt or truncated at the VP1) alone (odd numbered lanes) or with the plasmid pSKRH3C [46] encoding the FMDV 3Cpro (even numbered lanes) were analyzed by immunoblotting. Molecular mass markers (kDa) are indicated on the left. A negative control (No DNA) was included (lane 17). The different structural proteins are indicated on the right of the figure and were detected using guinea pig anti-FMDV O-Man antisera. Bound antibodies were visualized using the anti-guinea pig HRP-conjugated secondary antibodies and a chemiluminescence detection kit.