MOESM1 of Combining hydrophilic chemotherapy and hydrophobic phytotherapy via tumor-targeted albumin–QDs nano-hybrids: covalent coupling and phospholipid complexation approaches

Additional file 1. The additional information file include Characterization of the synthesized CdTe QDs, Preparation of PMT-conjugated BSA (PMT-BSA), Preparation of RSV-PC complex, Solid state characterization including FTIR Spectroscopy and DSC Thermograms, methodology of physicochemical characterization of dual drug-loaded BSA NPs including Drug loading and encapsulation efficiency, Particle size and zeta potential analysis, In vitro drug release, Physical stability study, Freeze drying and redispersibility, Morphological analysis, In vitro hemolysis and serum stability, In vitro cytotoxicity, Cellular-uptake study, the methodology of In vivo studies including Tumor growth biomarkers, Histopathological analysis, Immunohistochemical analysis, Quantification of the proliferative marker Ki-67 by image analysis technique and tissue localization of NPs. Immunogenicity of the nano-delivery system, Statistical analysis, the analysis of the FTIR study and body weight measurement study and the table of body weight average of mice groups. It also includes five figures which are: Figure S1. Physicochemical properties of the prepared RSV/PMT-BSA-QDs NPs (F2); size distribution diagram of non-targeted drug loaded BSA-QDs NPs (F2) (A) and their corresponding zeta potential distribution (B). Figure S2. A photograph illustrating the phenol sulfuric acid test of BSA (A) and Mannose-BSA NPs conjugate (B). Figure S3. 1H-NMR spectra of mannose-BSA, BSA and mannose revealing the presence of mannose protons in the spectra of mannose-BSA conjugate. Figure S4. DSC thermogram of RSV, RSV-PC complex, PMT, RSV/PMT-BSA-QDs NPs (F2) andMann-targeted RSV/PMT BSA-QDs NPs (F3) (A) and Fourier Transform Infrared (FTIR) spectra of RSV, RSV-PC complex, PMT, F2 and F3 (B). Figure S5. In-vivo anti-tumor efficacy showing change in body weights measurements of mice at indicated time-points along the experiment duration (C)