Additional file 1: of Inhibition of monocyte-like cell extravasation protects from neurodegeneration in DBA/2J glaucoma

Figure S1. CD45hi/CD11b+/CD11c+ monocyte-like cells infiltrate early in D2 glaucoma. At ~ 9 mo the majority of D2 eyes in our colony have experienced periods of high IOP. We have previously demonstrated that at this 9 mo time point there are early metabolic and transcriptomic differences in retinal ganglion cells that occurs prior to detectable axon degeneration [33]. Analysing ONH from D2 mice at this time point confirms that monocytes (CD45+/CD11b+/CD11c+) enter the ONH tissue during this early period of metabolic decline. These cells do not increase in either genetic control D2-Gpnmb+ or irradiated treat D2 ONHs (mice that are protected from neurodegeneration but still have a normal D2 front-of-the-eye disease [18]) suggesting that these infiltrating cells initiate a damaging cascade during glaucoma progression. n = 25 (D2-Gpnmb+), 45 (D2), 25 (irradiated D2). (TIFF 1162 kb