Mycoplasma transconjugant genomes and relative fitness.

(A) Mating experiments were conducted using the EnroR PG2E10 as donor and, the EnroS, gentamicin-tagged 5632G3 as recipient. Individual transconjugants were selected on solid media containing enrofloxacin and gentamicin and their genomes further sequenced by Illumina (see Materials and methods). Parental and transconjugant genomes are represented as concentric circles with PG2E10- and 5632G3-specific sequences indicated in orange and blue, respectively. The location of the gentamicin resistance marker (Gm) and of the Enro target genes parE, parC, gyrA and gyrB are shown on the periphery, with vertical black lines indicating the position of mutations relative to PG2 55–5. ICE relative positions in the 5632 genome are indicated by blue arrows. Strains and transconjugants are from inner to outer circle: PG2E10, T6-7, T6-3, T6-10, T6-1, T5-4, T6-4/8/9, T5-5, T6-5, T5-3, T5-1, T5-2 and 5632G3. (B) Transconjugants relative fitness (w) compared to the 5632G3 recipient strain is represented by histogram bars. The table summarizes the w values, the enrofloxacin MIC (in μg.mL-1), the number of PG2E10 fragments and the percentage of donor genome for each transconjugant. The percentage of donor genome was estimated by the total size of the PG2E10 transferred fragments divided by the size of the PG2E10 genome.