At left is shown the size-exclusion chromatogram corresponding to the final step of the purification of the full-length VanSA protein. In LDAO, the protein elutes as a single monodisperse peak. At right is a Coomassie-stained SDS-PAGE gel showing the purified VanSA; the asterisk indicates the dimer band.</p
Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The characte...
Supplemental Figure 2. Extracellular vesicles (EVs) from SW480 IMP1Null cells isolated by size-exclu...
(A) The degree of purity of SMA and LEN was assessed by SDS-PAGE analysis and deemed >95% pure, wher...
(A) Autophosphorylation of VanSA. Equivalent concentrations of full-length and cytosolic VanSA were ...
<p>The affinity-purified proteins were subjected to size-exclusion chromatography on a Superdex 200 ...
(A) Size-exclusion chromatogram of amphipol-reconstituted VanSA, demonstrating the presence of a sin...
<p>(A) Overview of the purification steps. (B) Coomassie blue stained SDS-PAGE gel and in gel fluore...
<p>DDM solubilized supernatant was subjected to purification in buffer containing DDM (a and b) or L...
<p>A = unincorporated common and variable strands, B = FLP, C = S1–S6 after size-exclusion purificat...
(A) Far UV-CD spectra of VL confirms correctly folded recombinant protein, displaying native β-sheet...
VanA-type resistance to glycopeptide antibiotics in clinical enterococci is regulated by the VanSARA...
<p>A two-step purification procedure was used to purify the proteins after being labeled with either...
Size exclusion chromatography traces of recombinant proteins (1mg/mL) with oligomeric states illustr...
<p>(A) The purified ΔH-domain protein (0.2 mg/ml) was loaded onto native-PAGE. M, protein marker; la...
Fractions produced from either differential centrifugation or size exclusion chromatography methods ...
Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The characte...
Supplemental Figure 2. Extracellular vesicles (EVs) from SW480 IMP1Null cells isolated by size-exclu...
(A) The degree of purity of SMA and LEN was assessed by SDS-PAGE analysis and deemed >95% pure, wher...
(A) Autophosphorylation of VanSA. Equivalent concentrations of full-length and cytosolic VanSA were ...
<p>The affinity-purified proteins were subjected to size-exclusion chromatography on a Superdex 200 ...
(A) Size-exclusion chromatogram of amphipol-reconstituted VanSA, demonstrating the presence of a sin...
<p>(A) Overview of the purification steps. (B) Coomassie blue stained SDS-PAGE gel and in gel fluore...
<p>DDM solubilized supernatant was subjected to purification in buffer containing DDM (a and b) or L...
<p>A = unincorporated common and variable strands, B = FLP, C = S1–S6 after size-exclusion purificat...
(A) Far UV-CD spectra of VL confirms correctly folded recombinant protein, displaying native β-sheet...
VanA-type resistance to glycopeptide antibiotics in clinical enterococci is regulated by the VanSARA...
<p>A two-step purification procedure was used to purify the proteins after being labeled with either...
Size exclusion chromatography traces of recombinant proteins (1mg/mL) with oligomeric states illustr...
<p>(A) The purified ΔH-domain protein (0.2 mg/ml) was loaded onto native-PAGE. M, protein marker; la...
Fractions produced from either differential centrifugation or size exclusion chromatography methods ...
Biopharmaceutical drugs are large, complex protein molecules derived from living cells. The characte...
Supplemental Figure 2. Extracellular vesicles (EVs) from SW480 IMP1Null cells isolated by size-exclu...
(A) The degree of purity of SMA and LEN was assessed by SDS-PAGE analysis and deemed >95% pure, wher...