U_L38-induced metabolic flux activation is mTOR independent.

After 24h incubation, conditioned serum free medium from confluent MRC5 cells transduced with an empty vector or UL38 was harvested for analysis. Media contained DMSO (+DMSO), 100 nm of rapamycin (+Rap) or 250nM of Torin-1 (TOR or +Torin1) as indicated. (A, E) Western blot analysis of treated EV and UL38 cells. (B, C, D, F & G) Changes in metabolic intermediates present in the conditioned medium were measured. Values are means ± SE (B-D n = 9, F-G n = 8). (*p<0.05, **p<0.01).