Immunohistochemical analysis of muscle extracellular matrix and associated proteins after tibialis anterior damage with CAMP or CTX.

Localisation (arrows) (A) and thickness (B) of collagen IV 5 days post administration. Similarly, localisation (arrows) (C) and thickness (D) of collagen IV 10 days post injury. Note: CTX damaged tissues show circumferential collagen compared to foci in CAMP treatment. Localisation (arrows) (E) and thickness (F) of laminin 5 days post injury. Localisation (arrows) (G) and thickness (H) of laminin 10 days post injury. I, localisation of dystrophin 5 days post injury (arrows). Note: dystrophin around centrally located nuclei in CTX-treated muscle. In contrast, incomplete dystrophin domain around CAMP-damaged muscle. J, intensity of dystrophin 5 days post injury. Localisation (arrows) (K) and intensity (L) of dystrophin 10 days post injury. M, localisation of nNOS 5 days post injury (no circumferential nNOS was detectable at day 5) and (N) thickness of nNOS 5 days post injury. O, localisation of nNOS 10 days post administration (arrows). Note: circumferential nNOS was only detectable in CTX-treated muscle and (P) intensity of nNOS 10 days post injury. Localisation of CAMP in damaged region at day 5 (Q) and 10 (R) (arrows). Data represent mean ± S.D. (n = 5 for each cohort). The p values shown are as calculated by One-way ANOVA followed by post hoc Tukey's test using GraphPad Prism (*p<0.05, **p<0.01 and ***p<0.001). CNT represents control.