Comparison of cell cycle determined by flow cytometry analysis among RAW macrophages 72 h cultures exposed OLZ (OLZ) at 0.03 μg/mL concentration and to phytohaemagglutinin (PHA), an natural antigen that trigger inflammatory macrophages activation.

(A) Representative graphics with cell phases: (G0/G1 = gap 1; S = synthesis; G2/M = gap2 and mitosis). (B) % of cells the control in at each stage (G0/G1 = gap 1; S = synthesis; G2/M = gap2 and mitosis) of the cell cycle compared by One-way analysis of variance (ANOVA), followed by the Tukey post hoc test. The different letters (i.e., A, B, C, D, E, F) indicate statistical differences in each treatment at p < 0.05. (C) Monolayer culture pattern of RAW macrophages microscopic optic analysis (×40, scale bar = 20 μm) in control (C) cells and cells exposed to OLZ and PHA. No-activated C-cells presented higher frequency of spheric cells typical of monocytes. Cells PHA and OLZ exposed presenting a macrophage spreading pattern that is observed in inflammatory-activated cells.