(A) Cells were treated with the indicated concentration of TGFβ1 for 16 h and then analyzed through Western blotting (n = 3). (B) Effect of TGFβ isoforms on collagen and CTGF mRNA expression in HESCs. Cells were treated with vehicle or TGFβ isoforms (ng/ml) for 4 h. The mRNA expression level of collagen, CTGF, and β-actin was analyzed through RT-PCR and densitometry (n = 2). *P P P < 0.001 versus vehicle treatment only (basal).</p
<p>(A) and (B) SVOG cells were pretreated with 10 μM SB431542 for 1 hour and then treated with 5 ng/...
<p>(A) CsA enhanced cell proliferation by MTS assay and RT-PCR analysis of PCNA. (B) CsA increased T...
<p>TGF-β treatment of cultured rat fibroblasts enhanced protein and mRNA levels of TGF-β receptor an...
(A and B) Cells transfected with control (CTL), CTGF, or HSP47 (HSP) siRNA were treated with vehicle...
<p>After treatment with TGF-β1 (1 ng/ml) alone or with PTHrP (100 nM) for 48 h, collagen I (138 kd) ...
Human CTGF was added to cells for 16 h. The cells were collected and collagen and HSP47 expression w...
<p>(A) SUIT-2 and KP-2 cells are transformed to a spindle-shaped morphology following treatment with...
<p>HTM cells were pretreated with 10 μM Y-27632 or 10 μM SB203580 for 30 min, and then stimulated wi...
<p>The expression of type III collagen in HSFs after 3 days (A), 1 week (B) and 2 weeks (C). Lane 1-...
<p>(A) Cells were stimulated with TGF-β1 (5 ng/mL) for the indicated times; then total RNA was extra...
<p>Effect on CTGF mRNA level: Four days after downregulation of CTGF by specific shRNA LEC were cult...
<p>(A) LX-2 cells and the isolated primary rat HSCs were treated with increasing concentrations of N...
<p>A. WF-A inhibits TGF-β1 induced increase in collagen polypeptides. HLFs were treated with DMSO (l...
(A) Measurement of pharmacological inhibitors on TGFβ1 signaling. Cells were pretreated with the ind...
<p>(A) Quantitative RT-PCR analysis for mRNA expression of α-SMA, type III and type I collagen genes...
<p>(A) and (B) SVOG cells were pretreated with 10 μM SB431542 for 1 hour and then treated with 5 ng/...
<p>(A) CsA enhanced cell proliferation by MTS assay and RT-PCR analysis of PCNA. (B) CsA increased T...
<p>TGF-β treatment of cultured rat fibroblasts enhanced protein and mRNA levels of TGF-β receptor an...
(A and B) Cells transfected with control (CTL), CTGF, or HSP47 (HSP) siRNA were treated with vehicle...
<p>After treatment with TGF-β1 (1 ng/ml) alone or with PTHrP (100 nM) for 48 h, collagen I (138 kd) ...
Human CTGF was added to cells for 16 h. The cells were collected and collagen and HSP47 expression w...
<p>(A) SUIT-2 and KP-2 cells are transformed to a spindle-shaped morphology following treatment with...
<p>HTM cells were pretreated with 10 μM Y-27632 or 10 μM SB203580 for 30 min, and then stimulated wi...
<p>The expression of type III collagen in HSFs after 3 days (A), 1 week (B) and 2 weeks (C). Lane 1-...
<p>(A) Cells were stimulated with TGF-β1 (5 ng/mL) for the indicated times; then total RNA was extra...
<p>Effect on CTGF mRNA level: Four days after downregulation of CTGF by specific shRNA LEC were cult...
<p>(A) LX-2 cells and the isolated primary rat HSCs were treated with increasing concentrations of N...
<p>A. WF-A inhibits TGF-β1 induced increase in collagen polypeptides. HLFs were treated with DMSO (l...
(A) Measurement of pharmacological inhibitors on TGFβ1 signaling. Cells were pretreated with the ind...
<p>(A) Quantitative RT-PCR analysis for mRNA expression of α-SMA, type III and type I collagen genes...
<p>(A) and (B) SVOG cells were pretreated with 10 μM SB431542 for 1 hour and then treated with 5 ng/...
<p>(A) CsA enhanced cell proliferation by MTS assay and RT-PCR analysis of PCNA. (B) CsA increased T...
<p>TGF-β treatment of cultured rat fibroblasts enhanced protein and mRNA levels of TGF-β receptor an...