HFA reduced osteoclast numbers in alveolar bone.

(A) Light microphotographs of H&E and immunohistochemically stained sections for TRAcP-5b. Images were collected close to the mid-root area of the first maxillary molar after 56 days of HFA application. Osteoclasts are identified as TRAcP-5b red cells in sections from different experimental groups. (B) Mean number of osteoclasts present in 5 consecutive sections of the ROI of the maxillary first molar. Each value represents the mean ± SEM of 6 animals (* Significantly different from Sham group; # significantly different from OVX group).