Slbp is required cell autonomously for retinal neuron differentiation.

(A, A’) Images from live timelapse recordings of Tg(atoh7:GFP)rw021 GFP-transgene expression in retinal neurons in control (A) and slbpty77e (A’) eyes at 30hpf. (B-B’) Frontal sections of 3dpf wildtype (B) and slbpty77e (B’) retinas showing anti-γ-tubulin labelled neurites/neuropil (magenta) and zpr1-expressing cone photoreceptors (green). The arrowhead points to a few remaining zpr1+ cone cells in the slbpty77e mutant eye. (C-C’) Frontal sections of 3dpf wildtype (wt) host retinas containing transplanted wildtype (C) or slbpty77e mutant (C’) GFP-labelled cells (green).(D-D’) High magnification images of transplanted cells in C-C’. Note the break in the plexiform layer in the retina (arrowhead) in the vicinity of the slbpty77e mutant cells. Abbreviations: nr, nasal retina. Scale bars: (A-C’) 50μm; (D-D’) 25μm.