IVM analogue potency.

A. Structures of IVM and the three analogues. Arrows indicate the synthesis reaction steps. MOMO represents a methoxymethyl ether group. A red broken border indicates the change in 5-OH configuration between IVM-2 and IVM-3. B. Example experiments in oocytes expressing α GluClRs that test IVM and analogues, IVM-1 and IVM-3. The IVM or analogue-induced current was normalised to the response elicited by a saturating concentration of glutamate (5 mM). C. Group bar plots comparing the potency between α and αβ (1:50) GluClRs for IVM and the three IVM analogues. *** p < 0.001, * p < 0.05. Numbers within the bars indicate the number of experiments (oocytes). Holding potential was −40 mV.