Additional file 7: of CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis

Impact of CSF1R antagonism on APC co-stimulatory expression is tissue-dependent. Mice were fed PLX5622 or control chow for 2 weeks, then infected via f.p. with WNV-NY (100 PFU). At 4 dpi, leukocytes were isolated from blood (A–C) and draining popliteal LNs (D–F). (A) Representative flow cytometry plots of CD86 expression on CD11c+CD45+-gated cells in the blood. (B) Quantification of percentages and (C) total numbers of CD86+CD11c+CD45+ cells in the blood. (D) Representative flow cytometry plots of CD80 expression on CD11c+CD45+-gated cells in the pLN. (E) Quantification of percentages and (F) total numbers of CD80+CD11c+CD45+ cells in the blood. For quantification panels, each symbol represents an individual control (black) or PLX5622 (red)-treated mouse, and bars indicate mean ± SEM. Data shown represent analysis from one experiment with four to five mice per group. Statistical significance was calculated using unpaired t test. For all data: ns, not significant at P < 0.05; *P < 0.05; **P < 0.01. (TIF 4465 kb