Additional file 1: of Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis

Figure S1.RFLP analysis of nuclease activities of three Cas12a (Cpf1) nucleases in rice protoplasts under different temperatures. Figure S2. RFLP analysis of SpCas9 activity in rice protoplasts under different temperatures. Figure S3. Measurement of rice protoplast transfection efficiencies using GFP construct at different temperatures. Figure S4. Three Cas12a nucleases generate slightly distinct mutation profiles at OsROC5 site which are unaffected by temperature. Figure S5. Mutation profiles by SpCas9 are largely unaffected by temperature. Figure S6. RFLP analysis of T0 mutant lines by AsCas12a. Figure S7. Late treatment of high temperature is ineffective on promoting LbCas12a-induced mutations in Arabidopsis. Figure S8. Analysis of AsCas12a-mediated mutagenesis at the TT4 locus in Arabidopsis protoplasts. Figure S9. Genotyping results of T1 maize lines. Figure S10. PAP1 expression at three different temperatures in the GUS control plants (WT). Figure S11. dCas12a-SRDX expression at three different temperatures in transgenic lines. Table S1. Guide RNA oligos. (PPTX 10187 kb