Additional file 1: of Intestinal dysmotility in a zebrafish (Danio rerio) shank3a;shank3b mutant model of autism

Figure S1. Insertion mutations in zebrafish shank3 orthologues produce unique restriction maps for genotyping. Figure S2. a Transverse 5 μm section of adult WT upper intestinal tissue stained with alcian blue and Eosin B (n = 6 for WT, n = 6 for shank3abΔC +/− and n = 6 for shank3abΔC −/−). b 40x magnification shows dense plicae that extend to the point of nearly occluding the luminal space. c, d In shank3abΔC +/− upper intestinal tissue, increased counts of goblet cells (black arrowheads) suggest inflammation. e, f Homozygous shank3abΔC −/− adults also show increased goblet cell count. Table S1. Oligonucleotides used for sgRNA synthesis. Table S2. Primers used for PCR and sanger sequencing. Table S3. % bead occupancy in WT and shank3abΔC+/− larvae at 3 h post-feed. Table S4. % bead occupancy in WT and shank3abΔC+/− larvae at 6 h post-feed. Table S5. % bead occupancy in WT and shank3abΔC+/− larvae at 12 h post-feed. Table S6. % bead occupancy in WT and shank3abΔC+/− larvae at 24 h post-feed. Table S7. Upper and lower GI rate of peristaltic period (seconds between contractions). Table S8. R script for Permutation. Table S9. shank3abΔC+/− injected with either Human SHANK3 mRNA short (32T) or long (5 T) isoform, 3–24 h post feed. (DOCX 4255 kb