Additional file 8: of CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis

PLX5622 treatment does not impact T cell activation in spleens of WNV-NS5-E218A infected mice. Mice were fed PLX5622 or control chow for 2 weeks, then infected i.c. with WNV-NS5-E218A (104 PFU). At 8 dpi, splenic leukocytes were isolated and analyzed by flow cytometric analysis. (A) Representative flow cytometry gating strategy for CD45+ cells. (B) Representative flow cytometry contour plots of CD4 vs CD8 expression on CD45+-gated cells. (C) Quantification of percentages and (D) total numbers of CD4+CD45+ vs CD8+CD45+ cells. (E) Representative flow cytometry plots of WNV-specific NS4B+ tetramer staining of CD8+CD45+ cells. (F) Quantification of percentages and (G) total numbers of NS4B+CD8+CD45+ cells. (H) Representative flow cytometry plots of CD69 expression on NS4B+CD8+CD45+ cells. (I) Quantification of percentages and (G) total numbers of CD69+NS4B+CD8+CD45+ cells. For quantification panels, each symbol represents an individual control (black) or PLX5622 (red)-treated mouse, and bars indicate mean ± SEM. Data shown represent analysis from one experiment with three to five mice per group. Multiple unpaired t test analyses indicate no significant difference among any of these populations. (TIF 7349 kb