Localization of autolytic factors in phosphate-starved cells.

Shown are the localization patterns of SdpA-mCherry (AM480, Pxyl::Pxyl-sdpA-mCherry), CrbA-mCherry (MAB247, Pxyl::Pxyl-crbA-mCherry), DipM-mCherry (AM208, Pxyl::Pxyl-dipM-mCherry), and SdpB-mCherry (AZ127, Pxyl::Pxyl-sdpB-mCherry) in cells cultivated for 24 h in M2G-P medium (scale bars: 3 μm). Synthesis of the fluorescent protein fusions was induced for 3 h (for DipM, SdpA, and CrbA) or 2 h (for SdpB) with 0.3% xylose prior to analysis. The demographs next to the images show the fluorescence profiles of a random subpopulation of cells sorted according to cell length (n = 200 for each strain).