ELISA OD values obtained testing sera from <i>Fasciola</i>–free (open circles) and <i>Fasciola</i>–infected (closed circles) cattle.

Each point in the figure represents the mean OD value (two replicates) obtained for one individual serum sample. IND = sera tested in indirect ELISA with rUS9-FhLAP antigen as target. US9 = sera tested in capture ELISA with mAb US9 coupled to ELISA plates and rUS9-FhLAP as target antigen. MM3 = sera tested in capture ELISA with mAb MM3 (MM3-ELISA; reference test) and Fasciola ESAs as target antigen. Horizontal lines represent the cut-off values (red dashed line: cut-off 1; blue line: cut-off 2). Cut-off 1 and cut-off 2 values for each ELISA were as follows: OD = 1.174 and 0.963 (IND); OD = 0.111 and 0.084 (US9); OD = 0.075 and 0.059 (MM3).