Additional file 3: of Circular RNA circNRIP1 acts as a microRNA-149-5p sponge to promote gastric cancer progression via the AKT1/mTOR pathway

Figure S3. (A). We performed a series of glycolysis detection experiments. We found that knockdown of circNRIP1 reduced lactate contents, glucose uptake and ATP production in MKN-45 and BGC-823 cells. However, the reduction in glycolysis activity was restored when we knocked down both circNRIP1 and miR-149-5p. (D). The extracellular acidification rate (ECAR) was measured. Knockdown of both miR-149-5p and circNRIP1 rescued the reduced glycolysis rate and glycolytic capacity observed when knocking down only circNRIP1. All data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. (TIF 350 kb