Additional file 5: of Tolerogenic XCR1+ dendritic cell population is dysregulated in HLA-B27 transgenic rat model of spondyloarthritis

Figure S5. Number of XCR1− cDCs in 6-month-old B27-Tg rat MLN after TLR-7 stimulation. Low-density cells were isolated from 6-month-old NTG and B27-Tg rats MLN that had been fed 5 h before with the TLR-7 agonist R-848 to activate DC migration from the intestine to the afferent lymph. Control NTG and B27-Tg rats received PBS (A) The graph shows the absolute number of XCR1− cDCs among CD4− CD103+ cDCs in R-848- and PBS-fed NTG and B27-Tg rats. (B) The graph shows the ratio of XCR1− cDC numbers between R848- and PBS-fed conditions in NTG and B27-Tg rats. This experiment was repeated 5 times. Bars show the mean ± SEM. Data were analyzed by unpaired Student’s t test. (PDF 42 kb