Defect in replication fork regression increases abundance of C-circles and 5' C-overhangs.

(A) Western blot shows efficiency of RPA2 knockdown by siRNA. β-actin was used as a loading control. U2OS cells were collected 60h after transfection with siRNA. (B) Abundance of C-circles in RPA2-depleted cells. Error bars represent the mean ± SEM of three independent experiments. Two-tailed unpaired student’s t-test was used to calculate P-values. *Pt-test was used to calculate P-values. **Pt-test was used to calculate P-values. ***P<0.001. (H) Effects of Rad51 inhibitor B02 on abundance of 5' C-overhangs. C-overhangs abundance was expressed as a ratio between the native and denatured signals. Values were then normalized with C-overhangs in control cells (siNC) to obtain relative abundance. Experiments were repeated four times and the mean ± SEM was indicated.