Changes to sterol composition in AmB-resistant parasites.
- Andrew W. Pountain (4114570)
- Stefan K. Weidt (702682)
- Clément Regnault (6293867)
- Paul A. Bates (126749)
- Anne M. Donachie (176370)
- Nicholas J. Dickens (268234)
- Michael P. Barrett (167748)
Publication date
February 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
A) Percentage sterol composition, as determined by GC-MS. Error bars represent standard deviation of the mean, n = 3. B) UV absorbance spectra of sterol extracts. Ergosterol dissolved in n heptane (0.05 mg/ml) is used as a standard, and n-heptane as a blank. Representative of three independent biological replicates. C) Sterol biosynthesis pathway in Leishmania, showing the structures of sterols identified in wild-type and AmB-resistant parasites. Coloured boxes relate to the colour key for sterols in panel A. Cholesterol is shown with the carbons numbered as referred to in the text.</p
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